Mass Cytometry 101: How does CyTOF Mass Cytometry work? | Boon-Eng Teh, PhD

28 April 20

Presented by Boon-Eng Teh, PhD, Fluidigm

Abstract: Mass cytometry uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. Cellular targets are labeled with metal-tagged antibodies and detected and quantified by time-of-flight mass spectrometry. The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. There are 135 available detection channels ensure an ongoing ability to add more parameters, enabling researchers to not just perform deep phenotypic characterisation but also in-depth functional characterisation. All in a single experimental setup to fully study the complexity of biological systems at the single-cell level. During this live session, we will provide an overview of the engineering advantages, panel design and key research applications of CyTOF Mass Cytometry. Live discussion and Q&A to follow after presentation.

About Helios, A CyTOF System:
Whether investigating therapeutic response in a human subject or a mouse model of disease, getting the most information from every precious sample is a priority in clinical and research studies alike. Mass cytometry with Helios™ uses CyTOF® technology to enable deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers. Designed to significantly reduce signal overlap, mass cytometry empowers researchers to simultaneously interrogate cellular phenotypes, function and signaling status in higher dimension than traditional fluorescence methods.