Mass Cytometry and Single-Cell RNA-seq Profiling of the Heterogeneity in Human PBMCs | Tae Hyun Yoon

1 September 2021

Fluidigm Greater Asia Webinar Series with Guest Speakers from Asia Pacific region.

We begin the session with a technology introduction by Fluidigm and thereafter a presentation by our Guest Speaker: Tae Hyun Yoon from Hanyang University, Seoul, Korea. On top of his various positions and responsibilities, Prof Yoon leads CNGC - the Center for Next Generation Cytometry.

Prof Yoon will share on his work on mass cytometry and single-cell RNA-sequencing (scRNA-seq) used complementarily to investigate the heterogeneous interactions between silver nanoparticles (AgNPs) and primary immune cells.

Mass cytometry allows ultra-high parametric analysis to reveal the heterogeneous bio-distribution of the positively charged AgNPs in various cell types and finds that monocytes and B cells have higher association with AgNPs than other populations. Coupled with scRNA-seq data of these two cell types, he was able to demonstrate that each type has distinct responses to AgNP treatment. Analysis of single-cell dose–response relationships further reveals within-population diversity for the B cells and naïve CD4+ T cells.

The study demonstrates that complementary use of ultra-high dimensional mass cytometry and scRNA-seq is helpful for gaining in-depth knowledge on the heterogeneous interactions between immune cells and NPs and can be incorporated into future toxicity assessments of nanomaterials. Live discussion and Q&A to follow.