Powered by CyTOF® technology.

Live-cell barcoding with Cd-CD45 antibodies | Michelle Poulin, PhD

Sample multiplexing with barcoding approaches
(*This webinar first aired on September 23, 2020, at the 9th Annual Fluidigm Mass Cytometry Summit: Virtual. )

With CyTOF technology, sample multiplexing is easily achieved with cell barcoding approaches that label individual samples before staining and acquisition of the combined samples. Barcoding reduces sample preparation time while increasing experimental throughput and improving data consistency and quality.
Several options for cell barcoding are available including live-cell barcoding, which labels human white blood cell samples with a unique 3-digit isotope code, using cadmium-labeled anti-CD45 (a pan-leukocyte marker) antibodies.

In this recording you will learn:

• Why barcoding is advantageous to your experimental design
• The products offered by Fluidigm for this application
• The step-by-step method including data analysis
• Workflow tips and tricks

Michelle Poulin, PhD
Manager, Proteomics Field Applications